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c glabrata atcc 2001d  (ATCC)


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    Structured Review

    ATCC c glabrata atcc 2001d
    C Glabrata Atcc 2001d, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+glabrata+genomic+dna/Candida+glabrata%3B+genomic+DNA/pmc10672136-45-5-7
    Average 93 stars, based on 7 article reviews
    c glabrata atcc 2001d - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Kar3Vik1 Mechanochemistry Is Inhibited by Mutation or Deletion of the C Terminus of the Vik1 Subunit
    Article Snippet: .. Full-length Kar3 and Vik1 sequences were PCR-amplified from C. glabrata genomic DNA (ATCC 2001D-5) and ligated into pCR2.1®-TOPO® (Invitrogen). .. Site-directed mutagenesis of Cg Vik1 was performed using QuikChange (Stratagene).

    Article Title: Kar3Vik1 Mechanochemistry Is Inhibited by Mutation or Deletion of the C Terminus of the Vik1 Subunit
    Article Snippet: .. Mutagenesis and Protein Expression and Purification—Fulllength Kar3 and Vik1 sequences were PCR-amplified from C. glabrata genomic DNA (ATCC 2001D-5) and ligated into pCR2.1 -TOPO (Invitrogen). .. Site-directed mutagenesis of CgVik1 was performed using QuikChange (Stratagene).

    Article Title: Neck Rotation and Neck Mimic Docking in the Noncatalytic Kar3-associated Protein Vik1
    Article Snippet: .. Plasmids encoding truncated C. glabrata Kar3 and Vik1 proteins ( A and supplemental Figs. S5 A and S7 A ) were constructed by amplifying relevant regions of C. glabrata genomic DNA (ATCC 2001D-5) by PCR and ligating them into either pET24d (Novagen) or a modified version of pET16b (Novagen) in which the Factor Xa cleavage site was replaced with an recombinant tobacco etch virus protease cleavage site. .. Cg Vik1 mutagenesis was performed using QuikChange (Stratagene).

    Article Title: Kar3Vik1 Mechanochemistry Is Inhibited by Mutation or Deletion of the C Terminus of the Vik1 Subunit
    Article Snippet: .. Mutagenesis and Protein Expression and Purification Full-length Kar3 and Vik1 sequences were PCR-amplified from C. glabrata genomic DNA (ATCC 2001D-5) and ligated into pCR2.1®-TOPO® (Invitrogen). .. Site-directed mutagenesis of Cg Vik1 was performed using QuikChange (Stratagene).

    Mutagenesis:

    Article Title: Kar3Vik1 Mechanochemistry Is Inhibited by Mutation or Deletion of the C Terminus of the Vik1 Subunit
    Article Snippet: .. Mutagenesis and Protein Expression and Purification—Fulllength Kar3 and Vik1 sequences were PCR-amplified from C. glabrata genomic DNA (ATCC 2001D-5) and ligated into pCR2.1 -TOPO (Invitrogen). .. Site-directed mutagenesis of CgVik1 was performed using QuikChange (Stratagene).

    Article Title: Kar3Vik1 Mechanochemistry Is Inhibited by Mutation or Deletion of the C Terminus of the Vik1 Subunit
    Article Snippet: .. Mutagenesis and Protein Expression and Purification Full-length Kar3 and Vik1 sequences were PCR-amplified from C. glabrata genomic DNA (ATCC 2001D-5) and ligated into pCR2.1®-TOPO® (Invitrogen). .. Site-directed mutagenesis of Cg Vik1 was performed using QuikChange (Stratagene).

    Expressing:

    Article Title: Kar3Vik1 Mechanochemistry Is Inhibited by Mutation or Deletion of the C Terminus of the Vik1 Subunit
    Article Snippet: .. Mutagenesis and Protein Expression and Purification—Fulllength Kar3 and Vik1 sequences were PCR-amplified from C. glabrata genomic DNA (ATCC 2001D-5) and ligated into pCR2.1 -TOPO (Invitrogen). .. Site-directed mutagenesis of CgVik1 was performed using QuikChange (Stratagene).

    Article Title: Kar3Vik1 Mechanochemistry Is Inhibited by Mutation or Deletion of the C Terminus of the Vik1 Subunit
    Article Snippet: .. Mutagenesis and Protein Expression and Purification Full-length Kar3 and Vik1 sequences were PCR-amplified from C. glabrata genomic DNA (ATCC 2001D-5) and ligated into pCR2.1®-TOPO® (Invitrogen). .. Site-directed mutagenesis of Cg Vik1 was performed using QuikChange (Stratagene).

    Purification:

    Article Title: Kar3Vik1 Mechanochemistry Is Inhibited by Mutation or Deletion of the C Terminus of the Vik1 Subunit
    Article Snippet: .. Mutagenesis and Protein Expression and Purification—Fulllength Kar3 and Vik1 sequences were PCR-amplified from C. glabrata genomic DNA (ATCC 2001D-5) and ligated into pCR2.1 -TOPO (Invitrogen). .. Site-directed mutagenesis of CgVik1 was performed using QuikChange (Stratagene).

    Article Title: Kar3Vik1 Mechanochemistry Is Inhibited by Mutation or Deletion of the C Terminus of the Vik1 Subunit
    Article Snippet: .. Mutagenesis and Protein Expression and Purification Full-length Kar3 and Vik1 sequences were PCR-amplified from C. glabrata genomic DNA (ATCC 2001D-5) and ligated into pCR2.1®-TOPO® (Invitrogen). .. Site-directed mutagenesis of Cg Vik1 was performed using QuikChange (Stratagene).

    Construct:

    Article Title: Neck Rotation and Neck Mimic Docking in the Noncatalytic Kar3-associated Protein Vik1
    Article Snippet: .. Plasmids encoding truncated C. glabrata Kar3 and Vik1 proteins ( A and supplemental Figs. S5 A and S7 A ) were constructed by amplifying relevant regions of C. glabrata genomic DNA (ATCC 2001D-5) by PCR and ligating them into either pET24d (Novagen) or a modified version of pET16b (Novagen) in which the Factor Xa cleavage site was replaced with an recombinant tobacco etch virus protease cleavage site. .. Cg Vik1 mutagenesis was performed using QuikChange (Stratagene).

    Modification:

    Article Title: Neck Rotation and Neck Mimic Docking in the Noncatalytic Kar3-associated Protein Vik1
    Article Snippet: .. Plasmids encoding truncated C. glabrata Kar3 and Vik1 proteins ( A and supplemental Figs. S5 A and S7 A ) were constructed by amplifying relevant regions of C. glabrata genomic DNA (ATCC 2001D-5) by PCR and ligating them into either pET24d (Novagen) or a modified version of pET16b (Novagen) in which the Factor Xa cleavage site was replaced with an recombinant tobacco etch virus protease cleavage site. .. Cg Vik1 mutagenesis was performed using QuikChange (Stratagene).

    Recombinant:

    Article Title: Neck Rotation and Neck Mimic Docking in the Noncatalytic Kar3-associated Protein Vik1
    Article Snippet: .. Plasmids encoding truncated C. glabrata Kar3 and Vik1 proteins ( A and supplemental Figs. S5 A and S7 A ) were constructed by amplifying relevant regions of C. glabrata genomic DNA (ATCC 2001D-5) by PCR and ligating them into either pET24d (Novagen) or a modified version of pET16b (Novagen) in which the Factor Xa cleavage site was replaced with an recombinant tobacco etch virus protease cleavage site. .. Cg Vik1 mutagenesis was performed using QuikChange (Stratagene).

    Virus:

    Article Title: Neck Rotation and Neck Mimic Docking in the Noncatalytic Kar3-associated Protein Vik1
    Article Snippet: .. Plasmids encoding truncated C. glabrata Kar3 and Vik1 proteins ( A and supplemental Figs. S5 A and S7 A ) were constructed by amplifying relevant regions of C. glabrata genomic DNA (ATCC 2001D-5) by PCR and ligating them into either pET24d (Novagen) or a modified version of pET16b (Novagen) in which the Factor Xa cleavage site was replaced with an recombinant tobacco etch virus protease cleavage site. .. Cg Vik1 mutagenesis was performed using QuikChange (Stratagene).



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    Image Search Results


    Minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations of the hydroalcoholic leaf extract of Vismia guianensis (EHVG) against Candida spp. strains.

    Journal: Antibiotics

    Article Title: The Anti-Virulence Effect of Vismia guianensis against Candida albicans and Candida glabrata

    doi: 10.3390/antibiotics11121834

    Figure Lengend Snippet: Minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations of the hydroalcoholic leaf extract of Vismia guianensis (EHVG) against Candida spp. strains.

    Article Snippet: C. glabrata (ATCC 2001) d , 3.125 , 6.25 , 2 , 0.25 , 16.

    Techniques:

    Effects of EHVG on time-kill curves. The effects of EHVG against Candida albicans SC5314 ( A ) and Candida glabrata ATCC 2001 ( B ) were tested at two different concentrations corresponding to the MIC or 2× MIC, then compared to untreated cultures (Control) or with cultures treated with Amphotericin B (AMPHO B; MIC—0.5 µg/mL and 2× MIC—1 µg/mL).

    Journal: Antibiotics

    Article Title: The Anti-Virulence Effect of Vismia guianensis against Candida albicans and Candida glabrata

    doi: 10.3390/antibiotics11121834

    Figure Lengend Snippet: Effects of EHVG on time-kill curves. The effects of EHVG against Candida albicans SC5314 ( A ) and Candida glabrata ATCC 2001 ( B ) were tested at two different concentrations corresponding to the MIC or 2× MIC, then compared to untreated cultures (Control) or with cultures treated with Amphotericin B (AMPHO B; MIC—0.5 µg/mL and 2× MIC—1 µg/mL).

    Article Snippet: C. glabrata (ATCC 2001) d , 3.125 , 6.25 , 2 , 0.25 , 16.

    Techniques: Control

    EHVG inhibited the adhesion of Candida albicans ATCC 90,028 ( A ), C. albicans SC5314 ( B ), and C. glabrata ATCC 2001 ( C ) in cultures treated with concentrations of ½ MIC and MIC when compared to untreated samples (control). The EHVG-treated cultures were also compared to those treated with the reference drugs Amphotericin B (Ampho B) or Fluconazole (Flu). (*) p < 0.05 differs from untreated control; (#) p < 0.05 differs from Amphotericin B.

    Journal: Antibiotics

    Article Title: The Anti-Virulence Effect of Vismia guianensis against Candida albicans and Candida glabrata

    doi: 10.3390/antibiotics11121834

    Figure Lengend Snippet: EHVG inhibited the adhesion of Candida albicans ATCC 90,028 ( A ), C. albicans SC5314 ( B ), and C. glabrata ATCC 2001 ( C ) in cultures treated with concentrations of ½ MIC and MIC when compared to untreated samples (control). The EHVG-treated cultures were also compared to those treated with the reference drugs Amphotericin B (Ampho B) or Fluconazole (Flu). (*) p < 0.05 differs from untreated control; (#) p < 0.05 differs from Amphotericin B.

    Article Snippet: C. glabrata (ATCC 2001) d , 3.125 , 6.25 , 2 , 0.25 , 16.

    Techniques: Control

    EHVG inhibited the formation of early ( A , C , E ) and mature ( B , D , F ) biofilms. The CFU/mL values were established in cultures of Candida albicans (ATCC 90028, A , B ; SC5314, C , D ) and C. glabrata (ATCC 2001, E , F ) after 48 h and 72 h. The samples treated with different concentrations of EHVG (1/4 and 1/2× MIC for early biofilms, and 2× and 4× MIC for mature biofilms) were compared to cultures treated with Amphotericin B (Ampho B) or fluconazole (Flu), and with the untreated control. (*) p < 0.05 in comparison to control; (@) in comparison to Fluconazole, and (&) p < 0.05 Fluconazole compared to Amphotericin B.

    Journal: Antibiotics

    Article Title: The Anti-Virulence Effect of Vismia guianensis against Candida albicans and Candida glabrata

    doi: 10.3390/antibiotics11121834

    Figure Lengend Snippet: EHVG inhibited the formation of early ( A , C , E ) and mature ( B , D , F ) biofilms. The CFU/mL values were established in cultures of Candida albicans (ATCC 90028, A , B ; SC5314, C , D ) and C. glabrata (ATCC 2001, E , F ) after 48 h and 72 h. The samples treated with different concentrations of EHVG (1/4 and 1/2× MIC for early biofilms, and 2× and 4× MIC for mature biofilms) were compared to cultures treated with Amphotericin B (Ampho B) or fluconazole (Flu), and with the untreated control. (*) p < 0.05 in comparison to control; (@) in comparison to Fluconazole, and (&) p < 0.05 Fluconazole compared to Amphotericin B.

    Article Snippet: C. glabrata (ATCC 2001) d , 3.125 , 6.25 , 2 , 0.25 , 16.

    Techniques: Control, Comparison

    Percentage of inhibition on EHVG-treated early ( A , C , E ) and mature biofilms ( B , D , F ) of Candida albicans ATCC 90,028 ( A , B ), C. albicans SC5314 ( C , D ) and C. glabrata ATCC 2001 ( E , F ) after 48 and 72 h, evaluated by MTT assay. Samples were treated with EHVG (1/4 and 1/2 for early biofilms, and 2× and 4× MIC for mature biofilms) and compared to untreated controls or the reference drugs Amphotericin B (Ampho B) and Fluconazole (Flu). (*) p < 0.05 compared to control. (#) p < 0.05 compared to Amphotericin B and (@) p <0.05 compared to fluconazole.

    Journal: Antibiotics

    Article Title: The Anti-Virulence Effect of Vismia guianensis against Candida albicans and Candida glabrata

    doi: 10.3390/antibiotics11121834

    Figure Lengend Snippet: Percentage of inhibition on EHVG-treated early ( A , C , E ) and mature biofilms ( B , D , F ) of Candida albicans ATCC 90,028 ( A , B ), C. albicans SC5314 ( C , D ) and C. glabrata ATCC 2001 ( E , F ) after 48 and 72 h, evaluated by MTT assay. Samples were treated with EHVG (1/4 and 1/2 for early biofilms, and 2× and 4× MIC for mature biofilms) and compared to untreated controls or the reference drugs Amphotericin B (Ampho B) and Fluconazole (Flu). (*) p < 0.05 compared to control. (#) p < 0.05 compared to Amphotericin B and (@) p <0.05 compared to fluconazole.

    Article Snippet: C. glabrata (ATCC 2001) d , 3.125 , 6.25 , 2 , 0.25 , 16.

    Techniques: Inhibition, MTT Assay, Control

    Reference strains tested.

    Journal: Antibiotics

    Article Title: The Anti-Virulence Effect of Vismia guianensis against Candida albicans and Candida glabrata

    doi: 10.3390/antibiotics11121834

    Figure Lengend Snippet: Reference strains tested.

    Article Snippet: C. glabrata (ATCC 2001) d , 3.125 , 6.25 , 2 , 0.25 , 16.

    Techniques: